Cell Culture Models in Preclinical Biopharmaceutics

For preclinical biopharmaceutical evaluations, various cell culture models have been established in our group. These comprise the Caco-2 cell line, a nowadays widely accepted model for the human intestinal epithelium, as well as models of prostate-, bladder- and mamma-carcinoma. Mainly, these cell culture systems are applied to elucidate preclinical biopharmaceutical aspects of specifically designed drug delivery systems. Among these our main focus is set on the estimation of cytoadhesion and cytoinvasion as well as the intracellular trafficking of drugs and their delivery systems. For a detailed characterisation of these issues, methods including flow cytometry, deconvolution microscopy and tailored fluorescence techniques, particularly in the area of immunofluorescence staining for co-localisation experiments, are applied. In addition, we have developed an acoustically driven mirofluidic cell chip to analyse particle-cell interactions under shear stress. However, our expertise also encompasses traditional horizontal and vertical setups to study transepithelial transport characteristics. Moreover, in collaboration with the Technical University of Vienna microelectronic biosensors coated with viable human cells have been developed. Currently, we try to evaluate formulations to improve the therapy of urinary tract infections as well as the diagnosis and treatment of bladder cancer.

Funding


1999 - 2002
FWF - P - 13513 MED
Accelerated drug development
by real time kinetic studies

2004
FFG - Nr. 80 83 89

Nahrungsergänzungsmittel
zur Behebung der Histaminintoleranz

2004 - 2008
EU - FP6

CellPROM - Cell programming by nanoscaled devices

2007 - 2008
FFG - Nr. 816418

A formulation containing Xyloseisomerase

2008 - 2009
FFG - Nr. 819695
Caricol Resorption